A MODIFIED METHOD FOR ESTIMATION OF FISH MUSCLE PROTEIN BY BIURET METHOD

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A biuret method for determination of protein in normal urine.

A biuret method has been developed which provides quantitative measurements of protein in normal urine without interference from drugs or pigments. This method is intended for use in monitoring clinical trials of new drugs-to detect nephrotoxicity. Protein is precipitated from duplicate samples of urine by addition of cold ethanolic phosphotungstic acid. The protein precipitates are separated b...

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Determination of protein in urine by the biuret method.

A method for the determination of protein in urine by means of the biuret reaction was described by Hiller (1) and by Hiller, McIntosh, and Van Slyke (2), in which pure biuret prepared by Kahlbaum was used as a standard for visual calorimetry. Since Hiller (1) the biuret method has been used for the determination of urinary proteins by Price (3) and by Lehmann (4), and has been widely used in m...

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Determination of Protein in Urine by the Biuret Method

A method for the determination of protein in urine by means of the biuret reaction was described by Hiller (1) and by Hiller, McIntosh, and Van Slyke (2), in which pure biuret prepared by Kahlbaum was used as a standard for visual calorimetry. Since Hiller (1) the biuret method has been used for the determination of urinary proteins by Price (3) and by Lehmann (4), and has been widely used in m...

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Assay of cerebrospinal fluid protein: a rate biuret method evaluated.

We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate fo...

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ژورنال

عنوان ژورنال: NIPPON SUISAN GAKKAISHI

سال: 1966

ISSN: 1349-998X,0021-5392

DOI: 10.2331/suisan.32.427